Biochem/physiol Actions

≥1 ng/ml kills Fas-sensitive cells

General description

Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG^®-tag at the N-terminus and expressed in HEK293 cells. Does not require enhancer for activity. Suitable for cytotoxicity assays. M.W. 32,000 - 35,000.

Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG^®-tag at the N-terminus and expressed in HEK293 cells. Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~37-42 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity. Useful for cytotoxicity assays.r>r>Fas Ligand (FasL/APO-1L/CD95L) is a ~37-42 kDa type II transmembrane protein belonging to the TNF family. Interaction between FasL and Fas induces apoptosis of Fas-sensitive cells. FasL is implicated in CTL-mediated-killing, activation-induced cell death, creation of immune-privileged sites and tissue homeostasis. The extracellular domain of human FasL can be cleaved off by a metalloprotease, generating soluble FasL (sFasL). Circulating sFasL can be detected in serum of patients with melanoma and certain types of lymphoma.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ashkenazi, A., and Dixit, V.M. 1998. Science281, 1305.Nagata, S., 1997 Cell88, 355.Hahne, M., et al. 1996. Science274, 1363.Bellgrau, D., et al. 1995. Nature377, 630.Lowin, B., et al. 1994. Nature370, 650.Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 4409.

Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~35 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity.

Packaging

5 µg in Plastic ampoule

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute in 50 µl PBS to yield a final stock concentration of 100 µg/ml. Further dilute in culture medium with 5% FCS immediately prior to use.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)